EP-022 - SELECTION OF COMPARISON DRUGS FOR ASSESSING THE CARDIAC PROTECTIVE PROPERTIES OF DRUG CANDIDATES.

D. Salimgareeva¹, S. Nurlanova¹, I. Bashirov², A. Vasilchenko³, A. Samorodov³; ¹Bashkir State Medical University, Ufa, Russia, ²Izhevsk State Medical Academy, Izhevsk, Russia, ³Ufa Law Institute of Ministry of Internal Affairs, Ufa, Russia.

Background: In connection with the widespread practice of myocardial revascularization, the issue of pharmacological protection of the myocardium in order to prevent the development of ischemia-reperfusion syndrome becomes quite relevant.
This research was conducted to select comparator drugs at the stage of evaluating the cardioprotective properties of drug candidates.

Methods: The work was carried out on adult male laboratory rats. The average weight was 254±12.7 g. Creatine monohydrate (C4H9N3O2) was enterally feed at a dosage of 2.0 g/kg of animal weight mixed with feed. The study was carried out using the Langendorff apparatus for perfusion of an isolated heart, the heart is placed under conditions of total ischemia followed by reperfusion. This technique is the best way to assess the resistance of the heart to ischemic damage and the effects of reperfusion. The study was approved by the ethical committee of Bashkir State Medical University.

Results: Based on the data obtained, enteral feed of creatine monohydrate to rats has a beneficial effect on the ability of the heart to tolerate ischemic damage, as indicated by both a longer maintenance of the experimental sample of activity (120 min. vs. 5 min. in the control group) and a greater myocardial contractility, as indicated by greater pulse pressure in the left ventricle while maintaining contractility (10 mmHg vs 4.5 mmHg).

Conclusion: Thus, the use of creatine monohydrate contributes to the preservation of the properties of the heart after a period of ischemia. This substance can be successfully used as a reference drug in the study of the cardioprotective properties of potential drugs.